Journal: Cancer Research

Article Title: Molecular Inversion Probe Analysis of Gene Copy Alterations Reveals Distinct Categories of Colorectal Carcinoma

doi: 10.1158/0008-5472.can-06-0595

Figure Lengend Snippet: Figure 2. Heat map of cluster from significant gene copy alterations in colorectal cancer cell lines and carcinoma samples. Unsupervised hierarchical clustering was done on the data set of significant gene copy regions as determined by CLAC. The dendrogram from the clustering algorithm was used to generate a heat map, graphically depicting the relationship among the colorectal cancer cell lines. The heat map of the data uses a green (losses) versus red (gains) color scale. Each column represents an individual MIP arranged by chromosome and physical location in increasing order from left to right. A, each row represents a separate colorectal cancer cell line. The two topmost branches are designated as clusters 1 and 2. A subcluster branch representing MSI cell lines is labeled as 1a. B, each row represents a separate colorectal carcinoma sample. The two topmost clusters are labeled as clusters 1 and 2.

Article Snippet: Colorectal cancer cell lines (CACO2, COLO320, DLD1, HCT116, HCT15, LOVO, LS174T, LS180, NCIH508, NCIH747, RKO, SKCO1, SW1116, SW403, SW48, SW480, SW620, and SW837) were obtained from the American Type Culture Collection (Manassas, VA) and grown under the recommended conditions according to the manufacturer’s instructions.

Techniques: Labeling

Journal: Cancer Research

Article Title: Molecular Inversion Probe Analysis of Gene Copy Alterations Reveals Distinct Categories of Colorectal Carcinoma

doi: 10.1158/0008-5472.can-06-0595

Figure Lengend Snippet: Figure 3. Overall frequency of deletions and amplifications of clusters 1 and 2 classification of primary colorectal carcinomas. The gene copy frequency between cluster 1 and 2 is separately plotted. Y axis, overall frequency of gene copy changes for individual MIP; X axis, location of probe as arranged by chromosome and their nucleotide location.

Article Snippet: Colorectal cancer cell lines (CACO2, COLO320, DLD1, HCT116, HCT15, LOVO, LS174T, LS180, NCIH508, NCIH747, RKO, SKCO1, SW1116, SW403, SW48, SW480, SW620, and SW837) were obtained from the American Type Culture Collection (Manassas, VA) and grown under the recommended conditions according to the manufacturer’s instructions.

Techniques:

Journal: Oncotarget

Article Title: MicroRNA-761 promotes the sensitivity of colorectal cancer cells to 5-Fluorouracil through targeting FOXM1

doi: 10.18632/oncotarget.20109

Figure Lengend Snippet: ( A ) The miR-761 expression in the colorectal cancer cell (HT29, SW480, SW620 and DLD-1) and normal colon epithelium cell line (FHC) was detected by qRT-PCR analysis. U6 was used as the internal control. ( B ) The expression level of miR-761 in the HT29 cell treated with miR-761 mimic was measured by qRT-PCR analysis. ( C ) The expression level of SW480 in the HT29 cell treated with miR-761 mimic was measured by qRT-PCR analysis. ( D ) The response of HT29 cells to 5-FU was increased after treated with the miR-761 mimic compared to the scramble-transfected cells. ( E ) The response of SW480 cells to 5-FU was increased after treated with the miR-761 mimic compared to the scramble-transfected cells. * p < 0.05; ** p < 0.01 and *** p < 0.001.

Article Snippet: Four colorectal tumor cell lines (HT29, SW480, SW620 and DLD-1) and one normal colon epithelium cell line (FHC) were purchased from the ATCC (MD, USA) and were keplt in the Dulbecco’s modified Eagle’s medium (DMEM). miR-761 and scramble, pcDNA-FOXM1 and control vector were synthesized from the GenePharma Company (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Control, Transfection

Journal: Oncotarget

Article Title: MicroRNA-761 promotes the sensitivity of colorectal cancer cells to 5-Fluorouracil through targeting FOXM1

doi: 10.18632/oncotarget.20109

Figure Lengend Snippet: ( A ) The miR-761 expression in the colorectal cancer tissues and the adjacent non-tumor tissues was determined by qRT-PCR. U6 was used as the internal control. ( B ) The expression of miR-761 was lower in the colorectal cancer samples compare to the non-tumor samples. ( C ) The miR-761 expression was lower in the colorectal cancer patients with low grade than in the patients with low grade. * p < 0.05.

Article Snippet: Four colorectal tumor cell lines (HT29, SW480, SW620 and DLD-1) and one normal colon epithelium cell line (FHC) were purchased from the ATCC (MD, USA) and were keplt in the Dulbecco’s modified Eagle’s medium (DMEM). miR-761 and scramble, pcDNA-FOXM1 and control vector were synthesized from the GenePharma Company (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Oncotarget

Article Title: MicroRNA-761 promotes the sensitivity of colorectal cancer cells to 5-Fluorouracil through targeting FOXM1

doi: 10.18632/oncotarget.20109

Figure Lengend Snippet: ( A ) The FOXM1 expression in the colorectal cancer tissues and adjacent non-tumor tissues was measured by qRT-PCR and U6 was used as the internal control. ( B ) The expression of FOXM1 was higher in the colorectal cancer samples than in the non-tumor samples. ( C ) The miR-761 expression was negatively associated with the expression of FOXM1 in the colorectal cancer tissues.

Article Snippet: Four colorectal tumor cell lines (HT29, SW480, SW620 and DLD-1) and one normal colon epithelium cell line (FHC) were purchased from the ATCC (MD, USA) and were keplt in the Dulbecco’s modified Eagle’s medium (DMEM). miR-761 and scramble, pcDNA-FOXM1 and control vector were synthesized from the GenePharma Company (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Control